Rebirth of the God-level Xueba

"Professor Yang, we now want to do a batch of comparative experiments to increase the success rate." Su Fan and Wang Liang only went to Yang Rui after preparing for a few days.

Although he is working in a laboratory, the person in charge of the laboratory is not easy to bother casually. If there is no dry goods in his stomach, it is easy to be bullied.

Yang Rui was sitting in the laboratory, and he didn't pretend that he knew everything.

Even if he started the sheep cloning project, he would not do every experiment personally. In fact, for such a large-scale project, his task is to supervise the completion of key points. As for the small parts, it is not worth investing energy.

Therefore, Yang Rui did not pay any attention to Su Fan and Wang Liangcai's problems, nodded calmly, and said, "Very good."

Originally, a comparative experiment was the only way to go. If Yang Rui had to teach this, the team would be too weak.

Su Fan has long been familiar with Su Fan's character and smiled and said, "Professor Yang, we have some ideas on the comparative test. I would like you to have a look."

"Oh? Which ones?" Yang Rui was still willing to deal with such specific problems.

"The first is the pretreatment solution. Gu Qiang uses 3% sucrose, which seems to be very effective, but he can't tell why. Considering that the sucrose solution may cause certain chemical damage to the oocytes, we think whether Need to do some research on the ratio of sucrose solution." Su Fan put forward the first question somewhat tentatively.

Yang Rui nodded and said, "That's right, what's the problem?"

He has automatically and consciously placed himself on the perspective of the problem solver.

When Su Fan heard there was a play, he quickly said: "I wonder if I can ask the laboratory to organize a team, or give us some additional funding to do this comparative experiment."

"Um..." Yang Rui hesitated to continue adding resources to Su Fan and Wang Liangcai's team.

Their progress is not slow, but such a steady and steady style is actually not very popular with Yang Rui.

Based on the current domestic economic situation, even Yang Rui’s laboratory cannot get the international average level of funding. It can only be said that the lower price level reduces the laboratory’s operating expenses, but many experiments point out that foreign exchange is used. , Funding is always tight. If every problem is overcome with resources, it will definitely be weak in the later stage.

"It's just a pretreatment solution, so I will start a research group and make a big fuss." Yang Rui meditated for a moment and expressed his opposition.

Changing to a local university to do some research on this seems to be a good topic. Many scholars probably don't mind investing a year or two here.

But from Yang Rui's point of view, there are too many steps to clone sheep, and the pretreatment solution is just a small point that can't be smaller. What can be done if the research is thorough.

Su Fan tried his best to reduce the expenditure and said: "In terms of pretreatment solution, an additional 100,000 to 200,000 yuan in funding should be almost the same."

If it is to save some, 200,000 yuan can still be relatively clear on the pretreatment operating fluid, and 100,000 yuan is a joke.

Yang Rui even shook his head.

200,000 yuan is not a lot. Although the total amount of funds he received is eight figures, each project and research group is spent at a running rate. Just a pre-processing operation fluid costs 200,000 yuan, not A good example.

In the final analysis, the right way to do projects in China is to save money.

"I think your development direction can be adjusted." Yang Rui thought about it for a moment, and said: "Your problem is that the sucrose solution will cause chemical damage to the oocytes. Therefore, you want to choose a solvent with as little damage as possible. is it?"

"Yes. We hope to increase the success rate as much as possible. The chemical damage caused by the sucrose solution is difficult to estimate. We are worried about the impact on the success rate in the later stage." Wang Liangcai hurriedly explained to Yang Rui.

Yang Rui nodded and said: "It is not difficult to understand your concerns in this regard, but the degree of chemical damage is difficult to verify."

"Yes..." Wang Liang was somewhat helpless.

"I think we should consider this issue from two perspectives." Yang Rui spoke slowly.

When it comes to pointing out the direction of the experiment, Yang Rui has never been stingy, nor has he too much scruples.

Seeing the direction from the chaos and driving in the fog was originally what scholars did.

If you do well, you will only get admiration, not doubt.

Wang Liangcai's spirit also came to the fore and listened to Yang Rui's words with bated breath.

Yang Rui pondered his tone and said: "First, let's talk about the pretreatment liquid. You can not only consider the reduction of chemical damage, but also the protection of oocytes."

Wang Liangcai and Su Fan looked at each other and didn't say a word. This is one of the obvious strategies, but the strategy may not be realized.

Yang Rui tapped the table twice with his fingers and decided to speak more clearly, saying: "If I were to do this experiment, I would definitely not use pure sucrose solution. I would consider giving the oocytes a more suitable environment, such as Said, fetal bovine serum is a good choice."

"Fetal bovine serum..." Wang Liang didn't expect that Yang Rui could really say something, and it sounded quite reasonable.

In the genetics laboratory, serum is also a commonly used reagent, and fetal bovine serum is not unfamiliar. However, if it had not been proposed by Yang Rui, Wang Liangcai and Su Fan, who were full of sucrose solution, would not have thought of it.

In fact, the entire laboratory, including Gu Qiang's group, has not tried to add fetal bovine serum to the operating fluid.

"Let's try it." Wang Liangcai was a little excited.

Judging from experience alone, adding fetal bovine serum is also a very reliable choice.

"Director, what about the second point?" Su Fan also feels reliable. He has been doing cell cloning for a long time, and he has used fetal bovine serum. Looking back now, I feel that all the indicators are in good agreement.

Yang Rui smiled and said, "The second point, I think your thinking still needs to be changed."

"Yes."

"Our goal is to clone sheep, um, cloned sheep asexually reproduced... In short, this project is not a commercial project for the time being. Therefore, we only need to make a cloned sheep in the end, which is infinitely high. The success rate is not very cost-effective."

Yang Rui seemed to be very relaxed, as long as there is a cloned sheep or something, Wang Liangcai and Su Fan's ears are almost helpless.

But what Yang Rui said is quite reasonable. If the funds are sufficient and the manpower is abundant, the bottom-up horizontal push is naturally the simplest and most feasible mode.

However, adequate funding and manpower are basically impossible.

There are very few scientific research projects that allow scholars to consume resources wantonly.

From Yang Rui's point of view, cloned sheep is a bottomless project.

Even in another three to four decades, people will still have a little understanding of the key points in cloning.

Historically, the Scots did more than 600 experiments to make a cloned sheep, Dolly. If their preliminary research is very sufficient, even out of insurance measures, two or three cloned sheep may eventually be born.

It can only be said that the appearance of the cloned sheep Dolly also has a certain element of luck.

Before it, there were only a few cloned sheep that were aborted, and countless wasted time and energy.

If Yang Rui does not want to spend the next ten years on the same project, it is imperative to adopt a more economical approach.

Thoroughly researching every key point is neither a correct choice nor impossible to achieve, and it is even more unnecessary.

As long as Yang Rui's laboratory can complete the sheep cloning project, the previous loopholes have not been studied clearly and the key points will naturally be checked and filled.

So many scholars from local universities, young people who just debuted, and laboratories for the poor in the third world have done this kind of work all their lives, and they are not tired of it, and they have to give others a chance.

Su Fan and Wang Liangcai also understood.

Yang Rui obviously did not approve of their step-by-step mode. Wang Liangcai couldn't help being a little anxious and said hurriedly: "Professor Yang, Director Yang, cloned sheep is a series of projects. If the success rate in the early stage cannot be guaranteed, there is no qualification to enter the later stage. ."

"Didn't Gu Qiang achieve nuclear injection?"

"We can't learn Gu Qiang's method. He knows it and doesn't know why." Wang Liangcai sighed.

Yang Rui said calmly: "Then know what is happening and not knowing why."

"what?"

"We are in the same laboratory, in the same project group, and divided into different research groups, just to try different paths. Since Gu Qiang has made one, let's use it, why spend the experience in opening up a parallel path. Come on? My suggestion, until the part of the verification, you only carry out the work of checking the missing and filling the vacancies, starting from the verification, continue to work hard."

Wang Liangcai was stunned when he heard it, but he felt a sense of empowerment.

"You are right, there is no need to entangle why the 5% concentration is used. The 5% concentration is easy to use." Wang Liang couldn't help knocking on his head.

Has anyone thoroughly studied an experiment?

Of course there are, but to achieve this step, it is usually done from the back to the front, that is, the future generations study the results of the predecessors.

Scholars who are truly doing pioneering research and standing at the forefront of science are like outlanders exploring the primordial rainforest. Who has the energy and time to explore the species and characteristics of each tree? Who can figure out the life under every inch of soil, or observe the movement in every tree canopy?

Doing scientific research is like driving in a foggy day. You can't stop because you are waiting to be hit by a car behind you. Don't expect much visibility, you can see the way under your feet, just don't just fall down.

Although Gu Qiang's experimental report lacks detailed analysis, one thing is okay. He at least proved that the road works.

"The success rate still needs to be there, but you don't need to chase the limit and solve the problem first." Yang Rui is equivalent to overthrowing the original thinking of Wang Liangcai and Su Fan, but the two are not unhappy.

You should listen to valuable suggestions. This is the minimum attitude of scientific research.

It is useless to engage in strength orders with the cell, and it is even more troublesome to get through with the funds.

Wang Liangcai thought for himself, and after a while, he said: "Since this is the case, let's repeat Gu Qiang's experiment first, and then extract the parts with obviously low success rate for in-depth research."

"In this case, Gu Qiang will lead the way for a while." Su Fan was slightly unwilling.

Wang Liangcai glanced at Su Fan and said, "It's not fast first. Cloning sheep is a big project. Just like long-distance running, we will implement the follow strategy."

Compared with Su Fan, Wang Liangcai's personality is obviously more calm, and his method is also the most able to take advantage of the two.

Moreover, compared to the stupid way of building foundations, the revised plan also takes into account the success rate and speed.

The most important thing is that Gu Qiang's advantage has also indirectly become the advantage of the two.

Yang Rui also nodded secretly. In this way, the efficiency of the laboratory can also be greatly increased.

No matter how good Gu Qiang's luck is, he can't really get the answer straight. Yang Rui is always needed to correct his direction from time to time~www.NovelMTL.com~ The most important thing is that the lack of theoretical reserves makes Gu Qiang's The ability to expand scientific research is weak.

For example, backtracking experiments, checking for missing vacancies, increasing the success rate, and improving the stability of experiments are not what Gu Qiang is good at.

But these abilities are indispensable for such a long project line as cloned sheep.

Wang Liangcai is on the contrary. He is not a scholar who is good at making surprises, but a stable long-distance runner. Putting down a little bit of insignificant face, what Wang Liang only got was an opportunity to reinforce and run fast.

"Professor Yang, we still want to apply for more time, especially at night." Wang Liangcai paused and said, "Can you give us all the time after the early morning?"

"If you apply for the night time, you must come every night." Yang Rui asked, "Can your body stand it?"

"I can take a two-hour nap at noon and another four hours after dinner. In addition, I can sleep for about two hours the next morning, no problem." Wang Liangcai said relaxedly, but all three of them knew. What a cruel perseverance is to spread the whole sleep time.

"Don't you need some... personal time?" Yang Rui has seen people who work hard and often ask experimenters to work hard, but it is rare that they actively ask for long working hours.

Wang Liangcai grinned and said, "No one in my family is in front of you. I want to do something in private time."

Yang Rui was speechless, shrugged, and said, "That's fine. Since your family is not in the capital, you can eat a small stove in the cafeteria. I will ask the nutritionist to prepare meals for you to ensure that it tastes good and has sufficient nutrition. By the way, I asked for a masseur a while ago, a national level one, and when someone arrives, I will allocate an hour to you every day."

Su Fan's envious saliva was about to flow down, but Wang Liangcai himself was at a loss.