Rebirth of the God-level Xueba

Yang Rui stayed in the nursing home and set up the equipment with Liu Shan.

There are many ways to isolate strains. In 1879, the French isolated high-temperature strains from the Seine River. After more than 100 years of development, the sample processing methods have long been diverse. The difference between times lies in simplified methods. Improve the yield.

Of course, the cost will increase accordingly.

Yang Rui first prepared a centrifuge, a machine commonly used in biological laboratories that can no longer be used to centrifuge water and mud samples.

Under the action of the centrifuge at 10,000 times per minute, the water sample will be directly separated from the impurities and supernatant enriched in bacteria.

The separated bacteria are of relatively high quality, and the supernatant will also be eluted out of the bacteria after passing through the filter membrane.

They are then cultured in the medium, and then they can be screened.

The principle of the mud sample is similar, except that it needs to be suspended in spring water, then centrifuged at a low speed to remove large particles, and then processed and enriched in a water sample method.

The whole process, in theory, is very simple, but the equipment is not simple.

A centrifuge that can rotate 10,000 times per minute is not cheap. It has a smaller capacity. It runs a dozen test tubes at a time in a group of ten minutes. It takes hundreds of kilograms of samples to complete the processing. It is unknown how long it takes.

The cultivation stage is also very expensive. The agar, glucose, peptone, etc. used in the culture medium are all more expensive than the same weight of food.

In addition, the cultivation conditions of 70 degrees or even above 80 degrees also put a huge pressure on the cost of cultivation.

Kang Hong helped Yang Rui bring equipment and materials, and after taking it apart, the only little flame of competition was extinguished.

He didn't want to compete in the first place, and isolating strains is not a new technology. The only thing that makes sense is what strains can be isolated.

Isolating powerful strains can naturally invent the thesis of cattle breaking, but if the isolated strains are only ordinary strains, it will be difficult to get in the introductory journals.

The results were ethereal like buying a lottery ticket, but Yang Rui invested such a high cost. When Kang Hong saw it, he felt that there was a lack of competitive value.

"Spending so much money to isolate thermophilic bacteria? Is it worth it?" Kang Hong asked, thinking about it.

"The instruments are not bad. After we increase the manpower, these instruments will still function." Yang Rui answered as he plugged in the cords and arranged them.

Kang Hong picked up a large box of filter membranes, placed it in the corner, and said, "Where are the consumables. These are all ultrafiltration membranes. They are all imported. How much do you need to use?"

Yang Rui said with an "en", "I originally planned to process a batch first, and then bring a batch back. These costs cannot be saved."

"It's too expensive. We can't afford it." Kang Hong tutted twice.

"If you are lucky, I guess there will be more left." Yang Rui said casually, and Liu Shan assembled the incubator.

There are many kinds of filter membranes, and the one that ordinary people are more familiar with should be the filter element in a household water purifier, which is also a kind of filter membrane, in the initial stage. These civilian products are all produced in laboratories. In the 1980s, they were of great value.

In fact, laboratories have higher requirements for materials and are not cheap at any time.

In order to isolate the desired thermophilic bacteria as soon as possible, Yang Rui was very willing to spend money. Kang Hong had no reason to make such a large investment, and he naturally couldn't understand it. It can only be attributed to the fact that the people of Beijing University have a lot of stupid money.

Yang Rui had no choice but to do so.

Isolating strains is a relatively common research task, but isolating the desired strains is not.

Historically, most antibiotics have come from the isolation of soil strains. In the past two to three decades, biologists have almost ruined soils all over the world, and the most successful of them is the terminator of tuberculosis. Waxman, he invented a systematic separation method. Then he got streptomycin, and then he won the Nobel Prize.

Waxman organized as many as 50 research dogs for this purpose and spent several years screening out more than 10,000 strains. I got the one I wanted...

Yang Rui couldn't afford to raise 50 experimental dogs, and he didn't have a few years to waste. Fortunately, he knew for sure which hot spring it was. As long as the number of separation was enough, he could always find the breed he wanted.

A simple laboratory was installed in most of a day.

Yang Rui casually grabbed a handful of soil from the ground, added some liquid, and put it into the centrifuge for inspection.

The 10,000-rpm centrifuge is very noisy, but Kang Hong is very excited.

"We also have one in our courtyard. It is locked with a wooden cabinet." Kang Hong stared at the machine in the transparent glass, and suddenly came.

Yang Rui understood: "The public laboratory in the school is like this."

"So is Peking University?" The current information is backward. The distance from Yunnan to Beijing is farther than from Beijing to Los Angeles, and it is about to catch up with Fuzhou to Taipei.

Yang Rui recalled, and said: "Almost, the administrator of the public laboratory only needs to have the instrument in good condition, and he doesn't care if it is convenient for you."

"That is to say, we must apply for a good machine once, at least two months in advance. If we can’t handle it, we still have to entertain guests. When it’s over, they say how many false invoices we issue, and what invoices are falsely issued, and we give them all I ate it." Any researcher, when it comes to the use of equipment, is full of fire.

When Yang Rui was a graduate student, he was also afraid of using instruments in public laboratories. Of course, what he was most afraid of was borrowing instruments from other laboratories.

This kind of pain, everyone who has spent half a year in the laboratory, has a deep understanding. Yang Rui tried every means to make money and built his own laboratory. Half of the reason was to avoid the reappearance of the dilemma.

If it's just to satisfy personal expenses, where does he need such trouble, buy a 2,000 yuan monkey ticket, wait until it is almost abandoned to change the house, and casually become a 21st century billionaire.

However, being just a billionaire, he did not decide his own research direction, and it was interesting to conduct his own experiments.

If there is no huge pressure and personnel struggle, as the head of a ** laboratory, do the scientific research you want to do, and really do it, it is quite a sense of accomplishment.

Kang Hong didn't have Yang Rui's experience, and didn't know how to get rid of the current restrictions. He just asked enviously, "Where do you get the equipment? Isn't it provided by Peking University?"

"Count the Hong Kong company funding."

"Hong Kong people are really rich." Kang Hong asked, "Where did you meet them?"

"I wrote the paper, they are interested... Well, the centrifuge stopped, and it looks good." Yang Rui took this to leave and get busy.

Liu Shan smiled at Kang Hong and started to lay hands on Yang Rui.

Yang Rui slowly got serious.

It is not worthwhile to isolate ordinary strains, but if you isolate extreme thermophilic bacteria, and then isolate the heat-resistant polymerase inside, this can be considered a good paper.

"" with an impact factor of 4.0 is about, if the target is lowered a little bit, it must be no problem.

In theory, it makes sense to spend 600,000 yuan on such a paper.

Although the cost-effectiveness is reasonable, considering that it is only the necessary steps for the thesis, it is not a disadvantage.

Kang Hong saw them getting busy, so he had to say goodbye.

Yang Rui and Liu Shan finished their preparations only in the afternoon when the weather cooled down.

"I haven't come back yet, maybe it was camping outside. What shall we eat at night?" Yang Rui looked at his watch. Camping was planned. The high-temperature hot spring was in an undeveloped state, and only a simple geological survey was done for one day. It is likely that the round trip will not be in time.

Liu Shan tilted her head and thought for a while, then raised her hand and said, "Eat instant noodles."

"Instant noodles? Well, it happens to match hot spring eggs." Yang Rui tidied up and went to the cafeteria to buy eggs and instant noodles.

At this time, instant noodles are still fresh products that old cadres want to taste.

"Where to eat?" Yang Rui asked, "I'll find a waiter and let him scald the eggs for us."

"Let's go together." Liu Shan carried a small bag, blinked, and said: "Let's make hot spring eggs by ourselves. You can soak in the hot springs and eat instant noodles and eggs at the same time."

Yang Rui said in surprise: "Men and women have separate baths."

"That little courtyard is empty." Liu Shan pointed to the courtyard at the foot of the mountain not far away.

A sign of "Superior Hot Spring Room" hung at the entrance of the courtyard, which is a small courtyard built around the hot spring pool. The introduced hot springs are steaming with heat and belong to the tenants alone. They can be soaked during the day and night without the use of multi-person changing rooms.

Yang Rui said, "They are only given by officials at or above the department level. I can't use Li Zhangzhen's identity certificate."

Yang Rui had obviously tried it. The staff of the nursing home insisted on their own opinions, and Yang Rui did not insist on it.

After the tourism resources are fully developed, there is nothing rare in such a hot spring room. It is nothing more than drawing out a pool of hot spring water and more pipes for water supply and drainage, which can charge hundreds of yuan more for the room, which stimulates countless hot spring resorts to devote themselves to this. .

But as far as the current state of resource development is concerned, the hot spring itself is not rare, but the construction cost has become a bottleneck. In addition, the sanatoriums for the development of hot springs are all fully funded units, and they do not have the urge to make profits. After the construction of one place, except for repairs and repairs, almost no new investment is given.

This is a small courtyard with a hot spring in it, which is a very fresh experience in Tengchong. When the number is scarce, it is natural to keep ~www.NovelMTL.com~ carefully to avoid the sudden arrival of senior leaders.

Liu Shan tightened the small bag in front of her chest and said: "There is no one inside, let's sneak in."

Then, Liu Shan took the lead, bypassing the lights at the small intersection and picking up from the low wall behind.

The sanatorium made by poor people for veteran cadres didn't even consider that some residents could climb up the wall.

Yang Rui looked down for a few seconds, gritted his teeth, "What are we afraid of", he followed and turned in.

When he turned into the small courtyard, Yang Rui was stunned.

Under the bright moonlight, Liu Shan was stretching her waist and taking off her coat.

In the dimness, Yang Rui saw beautiful curves, and heard fine sounds in his ears.

……(To be continued. If you like this work, you are welcome to come to the starting point to vote for recommended votes, monthly tickets, your support is my biggest motivation. For mobile phone users, please go to m to read.)

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