Rebirth of the God-level Xueba

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Yang Rui silently planned his project. The assistants around him only saw him reading the literature, in a daze (reading the literature), doing experiments on his own, and didn't know what Yang Rui was doing.

At this point, Yang Rui is already like a normal laboratory leader.

In fact, Yang Rui's work in the past six months has been all about preparation.

First of all, it is the expanding scale of Huarui Lab. The full name is polymerase chain reaction, which is a molecular biology technology.

In 1984, as long as anything was associated with a "molecule", it entered the high-tech field like the "nano" of the 21st century.

High is high cost, and new is new to new instruments.

Since the establishment of Huarui Laboratory, the equipment cost can exceed 1.5 million US dollars, which is Yang Rui's dividend for more than two quarters, which is equivalent to one-third of Yang Rui's income.

Anyone who is a rich man comes here, and when he sees such expenditure, he will be scared to pee.

This is pure investment, and it is very mysterious whether it can be recovered. Although Yang Rui has proved himself once, it is still difficult to say whether he will win if he is caught in the second scientific research competition.

Fortunately, there is no boss on Yang Rui, so he can prepare for it freely. Otherwise, don't look at the academician Cai of Peking University who value him so much, but if Yang Rui says that he wants to buy equipment with 1 million US dollars, it is also for free.

In the scientific research world, those with low status should do projects that require less resources. Otherwise, they will be irresponsible and do not have the life of a princess. Don’t expect your tutor and principal to treat you like a dad. The princess’s disease is terminally ill. Even if it is not cured, it is still necessary to try to control and improve the quality of life a little bit.

In addition to the hard and soft conditions of the Huarui laboratory, Yang Rui went to Tengchong before doing high-temperature polymerase, all of which were also preparations for the project.

But his preparations did not tell anyone the need.

Huang Mao, who made the high-temperature polymerase himself, didn't know the use of the high-temperature polymerase he made.

This is a situation often encountered by people engaged in research, anyway, if it is done, it is even a result.

It was also because he was not in a hurry to use it, Yang Zhi asked Huang Mao to post his thesis, and he did not hesitate to agree.

And under the heat-resistant polymerase, it is serious.

It can be complicated or simple. The complexity is the easiest for users, the so-called "automatic", students 20 years later, almost use this, put the material in, press two keys on the machine, and then wait Just spit it out.

Simple to the user is complicated. Each cycle must be supplemented with polymerase. The reason for supplementing with polymerase is that each cycle has a denaturation process. Through high temperature, the double-stranded DNA is dissolved. Open, this is a prerequisite for copying.

Before the thermostable polymerase was found, the general polymerase could only withstand a temperature of 37 degrees, the DNA would untie the double strands, and the polymerase would be dead.

Normal DNA is composed of two strands entangled together. The process of using polymerase to copy the two strands separately and then put them together is the process of copying DNA.

In this case, DNA is like a girl who is covered with white phosphorus and put on safety clothes.

The polymerase responsible for replication is like a depleted man.

The process of copying DNA requires mating.

When a man wants to mate, he must first tear off his safety suit.

The safety suit was torn off, and the white phosphorus with low ignition point burned instantly, and the man with low heat resistance level was burned to death. Another man needs to be added later, because the white phosphorus has been burned out, this time the copy was successful.

However, this is only the first set of cycles.

When the man who completed the mating entered the second set of cycles, he was obviously going to be burned to death by the new burning chastity pants, so a third man was added in.

There are drawbacks to constantly adding men. First of all, the corpse is more difficult to handle, and the look and smell are not good, which affects the subsequent mating, accumulates a lot, and even squeezes space.

Therefore, the best thing to do is to find a man who can withstand high temperatures, and the "burning chastity pants" will appear in cycles without being affected.

For a practical one, this technology has practical value only if you find a man who can withstand high temperatures.

Mouris suffered a loss in this regard.

Yang Ruiguang avoids this one and can save half a year, but he also wastes time.

He didn't have the appropriate gene fragment in his hand.

To be precise, Yang Rui did not have sequence analysis of gene fragments.

Human genome sequencing refers to the sequence analysis of human gene fragments one by one.

Because to copy the gene fragment, you must first know what is in the gene fragment. In other words, you must know all the information about the girl in safety clothes. Has she ever learned judo? how tall? How powerful is it?

After confirming the sequence analysis of the gene fragment, the primers can be determined, and then the appropriate polymerase can be sent for replication. For example, if you meet a judo girl, you can send Putin instead of Abe. Although the latter has something to do with judo, it is not appropriate. Compatibility, still have to be choked to death without hesitation.

When Mouris did it, he was in a large company, and he did the job of cloning gene fragments himself, and naturally there was a variety of gene fragment analysis.

Yang Rui didn't have this condition, even in China, Yang Rui had to look for suitable genes in published papers.

This is easier said than done, mainly because you are afraid of falsified papers or stupid papers written by fools.

The so-called not afraid of ten thousand, just in case, there are many such things as false papers or wrong papers in China, not uncommon in the United States, and neighboring Japan is exposed every year.

If it is an ordinary paper, Yang Rui will not delve into the authenticity of other people's papers, and it will almost work.

But once a $3 billion project is successful, I don’t know how many pharmaceutical companies and biotech companies will be staring at it. Yang Rui can’t let his paper build on an unstable paper.

It is not a paper made by a laboratory closely related to it. If you do not do verification, there will be any problems at that time. For a $3 billion project, it will be a gap that attracts flies.

If it is the gene fragment analyzed by his company, Yang Rui can also review the experimental process. If it is the gene fragment analyzed in China, Yang Rui can also ask Academician Cai-the other party is willing to show the best, if not, he can also have a basic judgment.

However, Huarui Lab does not have such reserves.

Then you have to repeat the experiment yourself.

However, the work of gene sequence analysis can be handed over to the "Ion Channel Laboratory" of Peking University.

Because according to Yang Rui's design, genetic analysis was originally one of the main goals of the official laboratory.

The future "Human Genome Project" is a huge project costing hundreds of billions of dollars. Moreover, the gene sequences obtained by the Human Genome Project are all public and not profitable.

No matter how wealthy and wealthy Yang Rui is, he can only pretend to be pure in front of such a plan.

Of course, genes other than the "Human Genome Project" are still profitable, such as the Tyrannosaurus rex gene. You can find something to study, and nobody cares about you.

But in any case, these are plans for the future. For now, repeating the sequence of several gene fragments is purely basic research, and it is also a good way to train soldiers to leave it to the "Ion Channel Laboratory".

After carefully selecting several plasmid fragments, Yang Rui immediately opened several small projects to clone the target fragment sequence in the ion channel laboratory.

The newcomers Su Xiankai and Fan Zhenlong, eagerly expecting to prove themselves, took over the work without surprise.

Xu Zhengping was a little surprised that Yang Rui repeated the experiment so much, but he had a lot of work under his hands, and he couldn't take care of others.

The only problem is that the number of experimental dogs is not enough.

After working for more than a day, Su Xiankai couldn’t help but found Yang Rui and said, “Professor Xu has his students to help, the public assistant in the laboratory, He Quangui and Zhao Pingchuan do not come every day, they also have some regular jobs. , I need at least a dedicated assistant to do experiments."

"It should be. I will arrange someone to help you first. You can make a report, and I will go to Professor Cai to ask someone." Yang Rui's laboratory is also operational, and the two researchers who are editing are in place, and they are here. Time to recruit experimental dogs.

"Me too." Fan Zhenlong raised his hand at the other end of the laboratory.

"One person and one full-time assistant, I know." Although Yang Rui got the two staff to Peking University, it would take a long time for the school to arrange teaching posts for them. During this period, they naturally couldn't help themselves. To find the experimental dog, Yang Rui can only arrange it.

However, seeing his own independent laboratory rise, Yang Rui also has a different mind.

He deliberately asked the two of them to make a report. After finding a time for Academician Cai in the office, he rushed over, handed in the report, and said: "In order to do research on coenzyme Q10, with the help of Zelican, Hedong University in Hedong Province has set up a joint laboratory called the Zelican Hedong University Laboratory. There are many students in the laboratory, and they are all carefully selected from the biology department of Hedong University. Many people have done it. After two or three years of experimentation, I was thinking, can I find two people from the laboratory of Zelikon Hedong University and transfer them to my ion channel laboratory."

What Yang Rui had to do was naturally to transfer Yao Yue over.

Academician Cai was unclear, so he said strangely: "Can they agree to transfer from Hedong University? Teacher or student?"

"I want two students who have been doing experiments for a long time, so that they can get started when they come, and there is no need to train. The students in our school who will do experiments have the masters. You have to choose carefully...The other one, I think Fan Zhenlong and Su Xiankai are both transferred from outside school. Newcomers may not be willing to cooperate with them. The students in the laboratory of Zelikan Hedong University are familiar with them." Yang Rui The reasons are very abundant.

Academician Cai pondered and said, "There is no such precedent before."

Yang Rui smiled: "It has only been a few years since the college entrance examination has been restored~www.NovelMTL.com~ You have a precedent for doing so, who dares to make irresponsible remarks. Isn’t there that exchange student abroad? Let’s have two one-way exchanges, Hedong The university should not oppose it either."

It is a little troublesome to talk about this matter, and it is impossible for Academician Cai to agree with ordinary teachers. However, the status of the "Ion Channel Laboratory" in the minds of Academician Cai does not stop there. The coordination issues raised by Yang Rui made Academician Cai a little bit more concerned.

The current college students are still very arrogant. Peking University’s students are naturally more arrogant. Fan Zhenlong and Su Xiankai were selected by Yang Rui. They are not very young and have no outstanding results. If they meet two people who can’t speak. Peking University students delayed the progress of the experiment without knowing.

The harmony of the laboratory is invisible and intangible, but it cannot be ignored.

Thinking of this, Academician Cai asked: "How do you want to operate?"

"Is it okay to ask Zelecom to come forward? Send two students from the Hedong Laboratory of Zelecom to Peking University, and exchange one to two years. There is no need to change the student status. Let's make arrangements here and treat them as our own students. , It’s time to go to class, to have a meeting, to focus on the laboratory side, and let Zeleca pay a little bit of tuition and miscellaneous fees..."

"What if you are not satisfied?"

"Then just call it back."

"Well, it is necessary to obtain the consent of the students and their parents and write a written consent form."

"No problem, come to Peking University, what else is unhappy?" Academician Cai, whom Yang Rui said, touched his beard and laughed.

...