Black technology, starting from daily check-in

Under Jiao Hong's arrangement, soon Lin Yu walked into the GMP laboratory with her wearing a white mask and white overalls.

Ouyang Lei and Zhou Yang, two non-professionals, stayed outside and did not come in.

After entering, Lin Yu found that there was already a 20-member gene editing team waiting for him.

Seeing this, Lin Yu didn't talk nonsense, and said straight to the point: "I won't say anything superfluous. I think you all know the purpose of my visit today, so please follow my instructions."

"From now on, you form a team by yourself, a group of five, and a group of people. You use a blood cell separator to collect about 50ml of mononuclear cell suspension from the venous blood just sent."

"People in the second group, you enrich T cells, and the enrichment can be done by density gradient centrifugation."

"The separation of T cell subsets is performed by CD4CD8-specific antibodies or surface marker-coupled magnetic beads, and stimulated by CD3 and CD28 antibodies to activate and separate the cells."

"Three groups, you prepare auxiliary tools such as lentiviral vectors, cells, and culture media, and ensure that all water sources in the experiment are ultrapure water."

"Four groups, you are responsible for filling the vacancies, and go where you need them."

As the orders came out from Lin Yu's mouth, the researchers who were at a loss also began to operate.

Under the leadership of Lin Yu, the whole team is like a starting machine, running efficiently.

Seeing that Lin Yu commanded each group of people so skillfully, even Jiao Hong who was visiting was very surprised.

Is this the chairman of the board or the head of a large professional team?

This professional knowledge and the proficiency of the commander are too exaggerated.

If she hadn't seen it with her own eyes, it would be hard for her to believe that this was actually the chairman of their company.

Seeing that the whole laboratory has started to operate, Lin Yu also nodded in satisfaction.

The entire CAR-T cell technology is divided into three parts.

The first step is to isolate the T cells and activate them.

The second step is to edit the genes of these isolated T cells and upgrade them so that they can be precisely guided to find and kill cancer cells.

The third step is to cultivate a large number of upgraded T cells, and then inject them into the body of the cancer patient whose blood was drawn.

In the end, you only need to slowly observe the adverse reactions.

Although it sounds simple, the second step in the whole process is the most critical and important.

Especially gene editing, the core technology involved in it, except for Lin Yuneng's research and development, no one else will know it at all.

As if thinking of something important, Lin Yu quickly looked at Jiao Hong and asked, "Can the data on sterility and endotoxin in our laboratory meet the data requirements of the Pharmacopoeia?"

Hearing this, Jiao Hong came back to her senses and said without hesitation: "The high-quality ultrapure water used in our laboratory not only meets the data requirements, but also exceeds them."

"Ah That's good."

Lin Yu nodded and continued to pay attention to the whole experiment process.

Three hours later, the tasks of the first and second groups were completed one after another without any mistakes.

Seeing this, Lin Yu also breathed a sigh of relief, because the next step is the preparation of the most important CAR-T cells.

This step is the most critical and also the core technology.

Lin Yu came to the experimental table, with the assistance of the third and fourth groups, picked up the tools in his hand, and devoted himself to the preparation of CAR-T cells.

The preparation of CAR-T cells is to add a chimeric receptor to T cells through genetic engineering technology that can recognize tumor cells, activate T cells at the same time, and finally kill tumor cells.

Relevant genetic engineering techniques include lentivirus, gamma retrovirus, Sleeping Beauty transposon system or mRNA transfection, mRNA electroporation, etc.

And Lin Yu used slow virus.

Through it, Lin Yu can effectively integrate exogenous genes or exogenous shRNA into the host chromosome, so as to achieve the effect of persistent expression of the target sequence.

In fact, to put it bluntly, it is to use genetic technology to transduce the sequence capable of producing CAR into T cells.

As Lin Yu carried out gene editing, all the researchers' eyes were focused on Lin Yu with a serious expression, and they were fascinated by each of them.

In this state of extreme concentration, time doesn't pass too fast, and the sky has already completely dimmed.

Meanwhile, outside the laboratory.

The two bosses, Ouyang Lei and Zhou Yang, leaned against the wall with a bowl of spicy instant noodles.

Soon, the fragrance gradually became stronger, and the two began to sip the instant noodles, while looking into the laboratory from time to time, talking to each other.

"Huh, huh, hiss...Mr. Lei, how long do you think Director Lin and the others will take to finish?"

Zhou Yang blew on the hot instant noodles a few times, then stuffed it into his mouth impatiently, and said vaguely.

Hearing this question, Ouyang Lei, who was finishing his last mouthful of instant noodles, couldn't help but look at the time on his watch.

At this time, the watch showed that it was already around twelve in the morning.

"It should be soon, I remember the time when Lin Dong finished developing the BTK inhibitor yesterday, it seems to be at this time."

Putting the finished instant noodles under the trash can, Ouyang Lei hesitated for a moment, and said with some uncertainty: "However, the CAR-T cell technology this time may be a little more difficult, and it may take a few hours. "

It has been half a day since the afternoon.

The two of them didn't do anything outside, just waiting for the news, they were a little hungry, so they could only eat instant noodles to satisfy their hunger.

But now, Lin Dong and the researchers in the laboratory are still focusing on the research and development of CAR-T cell technology without eating for a long time.

This made the two feel a little embarrassed about the difficulty of these people.

Especially their chairman, as a boss-level figure, Lin Yu is still hungry and fighting on the front line himself.

This comparison somewhat made the two of them feel a little embarrassed.

But people are like iron, rice is like steel, and they can't do anything about it when they are hungry.

Soon, time continued to pass, and it came to one o'clock in the morning.

In the laboratory, Lin Yu cloned his artificially synthesized CAR element into a lentiviral vector to package the pseudovirus.

These pseudoviruses are then used to infect T cells isolated from patients, and then CAR-T cells are gradually produced.

After completing this last step, Lin Yu looked at the CAR-T cells in the petri dish, and couldn't help but smile brightly on his face.

And after seeing Lin Yu stop what he was doing, all the researchers present came to their senses one by one as if they had just woken up from a dream.

"I have good news for you all."

Putting the tools in his hand on the experimental table, Lin Yu turned around, looked at the researchers around him with a smile, and said slowly: "About CAR-T cell technology...we have succeeded!!"

Lin Yu's voice went from low to high, from a sudden pause to the final burst.

The mood of all the researchers present was immediately detonated between the cadence and the ups and downs!

boom!

After hearing this news, all the researchers in the entire laboratory just felt that their brains went blank.

After a brief silence, excited cheers erupted, as if the entire laboratory was about to be overturned.

"Successful! We succeeded!!"

"Hahaha, this is really like a dream!"

"It's actually...really successfully developed..."

"Woooooo, CAR-T cell technology has succeeded, they are saved, they are finally saved."

Faced with this exciting news, the excitement and joy on the faces of some young male researchers were beyond words.

They hugged each other, cheered and jumped, very excited.

Some emotional female researchers thought of something sad and burst into tears on the spot.

Even those steady middle-aged researchers were also muttering to themselves, which was very shocking.

Because this impossible thing happened to them, it really made them feel like they were daydreaming.

CAR-T cell technology, a problem that plagues all medical companies around the world, was developed by them just like that...

No, they didn't develop it.

It was developed by their chairman!

All the researchers present were well aware of this.

They are just playing around.

In addition, they have been observing the gene editing technology of their chairman almost the whole time, which is basically a complete assistance.

This kind of thing can be done by a professional team.

But even so, their chairman is willing to share the research results with them.

This kind of supreme honor, which is enough to make the world's top gene editing team envious, was so easily obtained by them...

Thinking of this, all the researchers feel unbelievable, but also full of gratitude to their own chairman.

And outside the laboratory.

Ouyang Lei and Zhou Yang, who had been paying attention to the situation inside, were equally excited when they saw all the researchers inside cheering.

"Beautiful! It's actually successfully developed!"

Thinking of the impact of CAR-T cell technology, even though Ouyang Lei was prepared, he still blushed with excitement and waved his clenched right hand fiercely.

"The problem that even the top genetic team in the United States has not broken through has been solved by our chairman..."

Zhou Yang also sighed in amazement.

If he hadn't seen it with his own eyes, it would be hard for him to believe that this kind of thing happened.

This is simply unbelievable, even, this can already be said to be a miracle.

Moreover, what Zhou Yang did not expect the most was that this miracle was created by the newly appointed chairman of Tianjing Company today...

inside the laboratory.

After a short period of excitement, everyone also began to quiet down, casting their fiery eyes on Lin Yu in the center.

Seeing this, Lin Yu also smiled slightly, picked up the petri dish on the experimental table, and said: "Everyone, please look, the cells in the petri dish in my hand are CAR-T cells inlaid with receptors by me."

"Currently, the number of them is still too small to kill a large number of cancer cells in patients, so we need to culture CAR-T cells in vitro so that the number reaches a therapeutic level, such as several hundred million. "

"At that time, you only need to reinfuse the cultured CAR-T cells into the patient, and they will function in the patient's body."

"For example, once cancer cells are found, CAR-T cells will not only kill them, but also start to proliferate themselves, creating a huge army of cancer killers to destroy cancer cells together."

"Of course, the CAR-T cells in my hand can only treat the patient whose venous blood was drawn. For other cancer patients, CAR-T cells need to be prepared again..."

After briefly introducing some details, Lin Yu handed the Petri dish in his hand to Jiao Hong who was beside him, and said with a smile, "I've finished what I need to say, and I'll leave it to you."

"Well, Dong Lin, don't worry, I will take care of everything seriously." Jiao Hong took the petri dish with her hands carefully and said with certainty.

Hearing this, Lin Yu just smiled and nodded.

Suddenly, he seemed to think of something, and hastily added: "By the way, before the patient is reinfused with CAR-T cells, if chemotherapy is given some time in advance to kill a large number of cancer cells in the body, the therapeutic effect will be maximized. "

"Okay, I know."

Jiao Hong held the petri dish that attracted the attention of all the researchers, and said in a tone of impatience: "If there are no other questions, then I will take someone to culture CAR-T cells in vitro first."

Seeing her impatient look, Lin Yu waved his hands dumbfoundedly, motioning for her to go.

Seeing this, Jiao Hong held the petri dish in both hands, and led all the researchers present to move to a dedicated cell culture area.

Soon, the group directly invested in the in vitro cultivation of CAR-T cells again.