Rebirth of the God-level Xueba

The active contact of the Americans, especially Dalbecco, gave great stimulation to the five groups including Zhang Leshan. (From Q pen i house u)

In order to welcome the arrival of the Americans, Zhang Leshan and others did not hesitate to increase the funding for pcr-related experiments.

For example, Director Zhao’s heat-resistant polymerase group even increased expenditure and manpower in order to increase the speed.

The biology department of the entire faculty is showing a thriving situation. Every time Dean Cha comes out of the office to inspect and sees the vitality of the newly established laboratory under his control, he is deeply pleased. He only feels that the entire scientific research system seems to be To be brought to life by yourself.

Director Zhao and others also feel very comfortable. Compared to doing research on their own, repeating experiments or reverse engineering always makes people feel better.

This kind of innovation is like a deep mountain and old forest that no one has ever visited. You may not get anything if you go in. The only certainty is that you have to work hard to get out, and you may even never get out. The possibility of coming out.

Repeating experiments or reverse engineering is simple. It is like traveling to a famous mountain and river. You can basically determine what you can get when you drill in. The only thing that needs to be done is to work hard, maybe even a little bit. I have to give some more tickets, that's it.

Compared with the deep mountains and old forests, the famous mountains and rivers developed by the predecessors are naturally more comfortable. If you can save the entrance fees and travel expenses, it would be even better.

The refreshment of Director Zhao and others lasted for at least half a month. By mid-February, Director Zhao’s own heat-resistant polymerase group first had problems.

"The polymerase made according to the literature can withstand high temperatures up to 45 degrees, and it can't be improved." Zhu Guanghui, the researcher in charge of the experiment, reported to Director Zhao in discomfort.

"Didn't you let you do it again?" Director Zhao, who had just returned from Dong Laishun, had a mouthful of greasy mouths, and he didn't care about the taste.

"I did it again and isolated the strains again, but after it was made, the high temperature resistance... increased by two degrees." Zhu Guanghui said quickly and urgently, and only lowered his words when it came to specific temperatures. Speed ​​is also anxious.

Director Zhao frowned, and after a while, said: "45 degrees, too much difference."

Zhu Guanghui blushed a little, and in a second he straightened his chest to explain: "I know that 45 degrees is a bit lower, but compared to the research on thermotolerant bacilli that we did before, this temperature has increased a lot."

"Before Yang Rui made heat-resistant polymerases, the best heat-resistant polymerases were less than 40 degrees Celsius. We have increased by at least 20% now." Another researcher next to him also said.

"Yang Rui made the heat-resistant polymerase above 90 degrees." Director Zhao only felt that the teeth were tight, as if shredded meat was stuffed in.

Zhu Guanghui's face blushed. He was afraid that Director Zhao said this before, but now he finally heard it.

"A little more time, we should be able to improve." Zhu Guanghui's voice seemed to be roasting meat, and it was low and thin.

"How much to improve?" Director Zhao asked.

"50 degrees should be no problem, **** good-looking novel:."

"50 degrees does not make any sense." The heat-resistant polymerase is the raw material for the PCR instrument and must be able to withstand the high temperature of 96 degrees. The polymerase below this temperature is nothing more than an ordinary polymerase.

If there is no heat-resistant polymerase, then we have to go back to the era of manual PCR and do DNA replication like mala Tang.

It can be said that the heat-resistant polymerase is an important part of the PCR instrument. It can even be said that the first generation of the PCR instrument is only due to the heat-resistant polymerase. The PCR technology without the heat-resistant polymerase is just a technology that can be used. That's it, it is called pcr0.1 at most.

Director Zhao stared at Zhu Guanghui with serious eyes.

Zhu Guanghui stumbled: "We still need time."

"We have time, how long do you need?" Director Zhao's expression did not ease, he knew there must be a problem, otherwise, the atmosphere in the laboratory would not be so good in the first one and a half months, especially the half month after the official start.

Zhu Guanghui really said: "We have discovered a few situations that we did not anticipate before. Now, before we overcome the difficulties, we can't determine the specific time."

"Give you another month, what can you do?"

Zhu Guanghui whispered: "One month is not enough."

"A month and a half?"

Zhu Guanghui shook his head slightly.

"Two months?"

Zhu Guanghui's head drooped.

"You say a number."

Zhu Guanghui thought for a long time and said, "For half a year..."

"Can you make the same heat-resistant polymerase in half a year?"

"Maybe we can make a usable heat-resistant polymerase." Zhu Guanghui's voice became lower.

Usable and the same are two concepts. As a kind of enzyme, heat-resistant polymerase actually has the best working temperature. The so-called heat-resistance usually means that it can work under high temperature conditions, that is, it can be used. However, it must work best under high temperature conditions. Good performance, this is very difficult.

Yang Rui has done enough preparations, especially with a clear direction, to make a useful polymerase.

But for Director Zhao's experimental group, this question is not so simple.

However, as the most important raw material of the PCR instrument, whether the heat-resistant polymerase is good or not will affect the effect of the final PCR instrument to a large extent.

Director Zhao said solemnly: "It's just maybe? Two days ago, you were still very confident, right?"

"We didn't expect that as the temperature rises, the problems we encounter will increase geometrically." Zhu Guanghui faced dejectedly.

Director Zhao darkened his face and said, "Let me see."

Zhu Guanghui hurriedly took out the experimental records, and then explained several key points encountered to Director Zhao.

Director Zhao is the person in charge of the laboratory, and his level of scientific research is not weak.

After watching for a while, Director Zhao found that Zhu Guanghui's judgment was correct.

"This is troublesome." Director Zhao shook the experimental record, thinking about funding and time.

Although it is reverse engineering and there are literature references, the specific experimental procedures, materials and details are not clear, and there are still countless kinds of crossover possibilities.

Director Zhao’s laboratory is not as good as Huarui in terms of conditions, capital is not as good as Huarui, and the direction and guidance rules are not as good as Huarui. The time they put in is far less than the time invested by Huarui, and they can’t do it. Of course.

If you travel to famous mountains and rivers, if potassium ion channel related research is to climb Mount Hua, the heat-resistant polymerase is roughly equivalent to climbing the Yulong Snow Mountain, which is not as hard and dangerous as Mount Hua, but it is the same hard and dangerous.

If you are well prepared for the snow-capped mountains in such a tourist area, healthy people can climb it. The only difference is the investment of time and energy.

Yang Rui spent half a year in preparation at the beginning, and half a year to do it. Huang Mao, who actually operates, is even a Mavericks-his current level is naturally not up to the Mavericks, but his experimental ability is already acceptable. Like the Mavericks.

Director Zhao is underestimating, so he can only handle it as Taishan, which will naturally estimate the wrong time and cause delays.

"Director, what do you do now?" In the laboratory, a researcher asked the question everyone wanted to ask.

In a laboratory, the biggest and most important person in charge usually doesn't need to do experiments in person. This is like the biggest and most powerful executive chef in a big hotel, who usually doesn't hold the spoon.

In addition to obtaining projects and funds, the person in charge of the laboratory is mainly to determine the direction and solve the problem, just like the executive chef who mainly decides how to prepare a dish and how to deal with problems.

In addition to this, he naturally has to supervise time and expenditures, just like the executive chef has to tighten the speed of serving dishes and restrict the use of raw materials.

Usually, the director of the laboratory can eat Donglaishun if he wants to eat Donglaishun, but when problems arise, he must make a decision.

Director Zhao is full of paste~www.NovelMTL.com~ No heat-resistant polymerase has been made in China before.

Before pcr, this was originally an unimportant branch of scientific research.

Therefore, from the perspective of scientific research, the heat-resistant polymerase was made from scratch, and the temperature of the heat-resistant polymerase was increased to more than 40 degrees. This is a very good achievement, and it is enough to fill the domestic gap. Wait, the domestic gap has been filled by Yang Rui. This is probably one of the reasons why some people don’t like Yang Rui. In the past, foreign countries made innovative achievements and copied from China. This is to fill the gaps. Now, Yang Rui does Innovative results have been produced and copied from China, but it is not useful.

Therefore, before the scientific research community reinvented a vocabulary, classic expressions such as filling the gaps in the country became difficult to use.

As far as the heat-resistant polymerase is concerned, Yang Rui actually made the high-temperature polymerase raw materials several years in advance. The capital he invested in this was not available to Director Zhao.

"Unexpectedly..." Director Zhao was lost in thought.

"Unexpectedly..." Several scientific research groups have fallen into contemplation one after another. For mobile phone users, please visit